Immobilization strategy for enhancing sensitivity of immunosensors: L-Asparagine-AuNPs as a promising alternative of EDC-NHS activated citrate-AuNPs for antibody immobilization
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- 作者：Ragini Raghav ; Sudha Srivastava ; sudha.srivastava@jiit.ac.in" class="auth_mail" title="E-mail the corresponding author
- 关键词：Covalent immobilization ; Dynamic range ; EDC&ndash ; NHS ; Gold nanoparticles ; L-Asparagine capped AuNPs ; Sensitivity
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 April 2016
- 年：2016
- 卷：78
- 期：Complete
- 页码：396-403
- 全文大小：2680 K
文摘
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Comparing strategies for covalent immobilization of antibody (Ab) for immunosensor.
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Asn–AuNP better candidate than EDC–NHS activated cit-AuNP for immobilizing Ab.
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Orthogonal immobilization of Ab with 88% active Ab for Asn–AuNP-Ab immunosenor.
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Asn–AuNP–Ab immunosensor sensitivity 2.5 times that of cit-AuNP–EDC–NHS–Ab.
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Immunosensor dynamic range: Asn–AuNP–Ab>cit-AuNP–Ab=cit-AuNP–EDC–NHS–Ab.