Fluorescence site-encoded DNA addressable hapten microarray for anabolic androgenic steroids

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• 作者：Nuria Tort ; J.-Pablo Salvador ; M.-Pilar Marco ; Ramó ; n Eritja ; Marta Poch ; Elena Martí ; nez ; Josep Samitier ; Josep Samitier ; Nuria Tort ; J.-Pablo Salvador ; M.-Pilar Marco ; Ramó ; n Eritja ; Ele
• 关键词：Anti-doping ; DNA chip ; DNA-directed immobilization (DDI) ; Fluorescence ; Food safety ; Hapten microarray ; Immunochemical screening ; Proof of concept ; Small organic molecule ; Steroid
• 刊名：TrAC Trends in Analytical Chemistry
• 出版年：2009
• 出版时间：June 2009
• 年：2009
• 卷：28
• 期：6
• 页码：718-728
• 全文大小：1646 K

文摘

We report a new strategy for immunochemical screening of small organic molecules based on the use of a hapten microarray. Using DNA-directed immobilization strategies, we have been able to convert a DNA chip into a hapten microarray by taking advantage of all the benefits of the structural and electrostatic homogeneous properties of DNA. The hapten microarray uses hapten-oligonucleotide probes instead of proteins, avoiding the limitations of preparing stochiometrically-defined protein-oligonucleotide bioconjugates.

As proof of concept, we show here the development of a microarray for analysis of anabolic androgenic steroids. The microchip is able to detect several illegal substances with sufficient detectability to be used as a screening method, according to the regulations of the World Anti-Doping Agency for sport and the European Commision for food safety.

The results that we show corroborate the universal possibilities of the DNA chip, and, in this case, they open the way to develop hapten microarrays for the immunochemical analysis of small organic molecules.