Inhibitory effect of imperatorin on insulin-like growth factor-1-induced sebum production in human sebocytes cultured in vitro

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• 作者：Yul-Lye Hwang^a ; ^b ; Myung Im^a ; Min-Ho Lee^b ; Seok-Seon Roh^c ; Byoung Wook Choi^d ; Sue Jeong Kim^a ; Kyung-Cheol Sohn^a ; Young Lee^a ; Young-Joon Seo^a ; Jeung-Hoon Lee^a ; ^e ; Chang Deok Kim^a ; ^{cdkimd@cnu.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Acne ; Imperatorin ; IGF-I ; Sebocytes
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：1 January 2016
• 年：2016
• 卷：144
• 期：Complete
• 页码：49-53
• 全文大小：579 K

文摘

Acne is a common skin disease that originates in the sebaceous gland. The pathogenesis of acne is very complex, involving the increase of sebum production and perifollicular inflammation. In this study, we screened the anti-lipogenic material and demonstrated its effect using cultured human sebocytes.

Main methods

Normal human sebocytes were cultured by explanting the sebaceous glands. To evaluate the anti-lipogenic effect, sebocytes were treated with test materials and ¹⁴C-acetate incorporation assay was performed.

Key findings

To screen the anti-lipogenic materials, we tested the effect of many herbal plant extracts. We found that Angelica dahurica extract inhibited the insulin-like growth factor-1 (IGF-1)-induced sebum production in terms of squalene synthesis in sebocytes. Furthermore, imperatorin isolated from A. dahurica showed remarkable inhibitory effect on squalene production as well as squalene synthase promoter activity. To investigate the putative action mechanism, we tested the effect of imperatorin on intracellular signaling. The results showed that imperatorin inhibited IGF-1-induced phosphorylation of Akt. In addition, imperatorin significantly down-regulated PPAR-γ and SREBP-1, the important transcription factors for lipid synthesis.

Significance

These results suggest that imperatorin has a potential for reducing sebum production in sebocytes, and can be applicable for acne treatment.