The mRNA of claudins is expressed in the endolymphatic sac epithelia

详细信息    查看全文

• 作者：Ai Matsubara ; ^{aimatsu@med.kagawa-u.ac.jp} ; Takenori Miyashita ; Terushige Mori ; Kosuke Akiyama ; Ryuhei Inamoto ; Nozomu Mori
• 关键词：Claudin ; Endolymphatic sac ; Paracellular transport ; Tight junction ; Endolymph ; In situ hybridization
• 刊名：Auris Nasus Larynx
• 出版年：2012
• 出版时间：August, 2012
• 年：2012
• 卷：39
• 期：4
• 页码：361-364
• 全文大小：319 K

文摘

Objective

Claudins are a family of membrane proteins which localize to tight junctions (TJs). Recent studies have shown that claudins can form pores for ions in the TJs and regulate the permeability of epithelial paracellular ion transport. The endolymphatic sac (ES) is a part of the inner ear, absorbing the endolymphatic fluid. ES dysfunction may result in endolymphatic hydrops. In this study, we focused on the paracellular transport and examined claudin mRNA expression in the ES epithelia.

Materials and methods

Total RNA was isolated from whole ES epithelia of rats by laser capture microdissection. RT-PCR was used to evaluate the expression of claudins. The expression of each claudin mRNA in the epithelial cells of rat ES was confirmed by in situ hybridization.

Results

RT-PCR indicated the expression of cldn2, cldn4, cldn6, cldn7, cldn9, cldn11, cldn12, and cldn14. The expression of these claudin mRNAs in the epithelial cells of rat ES was confirmed by in situ hybridization.

Conclusion

We demonstrated mRNA expression of multiple claudins in the rat ES epithelia. These results in the ES epithelia were consistent with a role of claudins in paracellular ion transport.