Expression of thiazide-sensitive Na⁺–Cl⁻ cotransporter in the rat endolymphatic sac

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• 作者：Kosuke Akiyama ; Takenori Miyashita ; Terushige Mori ; Ryuhei Inamoto ; Nozomu Mori
• 关键词：Endolymphatic sac ; Thiazide sensitive Na⁺– ; Cl⁻ ; cotransporter (TSC) ; Endolymphatic hydrops ; Ion transport ; Aldosterone ; Endolymph ; Inner ear ; Cochlea ; Electroneutral cation-coupled Cl⁻ ; cotransporters ; Co
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2008
• 出版时间：11 July 2008
• 年：2008
• 卷：371
• 期：4
• 页码：649-653
• 全文大小：476 K

文摘

The endolymphatic sac (ES) is a part of the membranous labyrinth and is believed to absorb endolymph. It has been well-established that the endolymph absorption is dependent on several ion transporters in a manner similar to that in the kidney, and the ES is regulated by hormones such as aldosterone and vasopressin that also affect on the kidney. The thiazide-sensitive Na⁺, Cl⁻ cotransporter (TSC) is an electroneutral cotransporter specific to the kidney that plays an important role in absorption of NaCl in renal tubules. In the inner ear, TSC expression has never been examined. The expression of TSC in the rat ES was examined by RT-PCR, in situ hybridization and immunohistochemistry. These analyses indicated that TSC genes and proteins were expressed in the rat ES. In contrast, it was not observed in the rat cochlea by RT-PCR. This is the first report confirming the expression of TSC in the ES.