Establishment of a heterotypic 3D culture system to evaluate the interaction of TREG lymphocytes and NK cells with breast cancer
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- 作者：Tanya N. Augustinea ; tanya.augustine@wits.ac.za" class="auth_mail" title="E-mail the corresponding author ; Thé ; rè ; se Dix-Peekb ; Raquel Duarteb ; Geoffrey P. Candyb
- 关键词：2D ; two-dimensional ; 3D ; three-dimensional ; BPCM ; basal-phenotype culture model ; ER ; oestrogen receptor ; ECM ; extracellular matrix ; EGFR ; epidermal growth factor receptor ; GFRM ; growth factor-reduced Matrigel ; HER2/neu ; human epidermal growth factor receptor 2 ; IL ; interleukin ; IFN-γ ; interferon-γ ; LPCM ; luminal-phenotype culture model ; MUC1 ; mucin 1 ; NK cells ; Natural Killer cells ; PBMCs ; peripheral blood mononuclear cells ; PR ; progesterone receptor ; TIL ; tumour infiltrating lymphocyte ; TNF ; tu
- 刊名：Journal of Immunological Methods
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：426
- 期：Complete
- 页码：1-13
- 全文大小：2772 K
文摘
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Developed a 3D culture system to study immune mediation of breast tumour progression
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Natural Killer & regulatory T cells were cultured with tumour cell lines in Matrigel
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3D model feasible for immunocytochemistry, RNA extraction & cytokine analysis
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Tumour morphology, expression of biomarkers and CCL4 varied under immune mediation