Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit
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- 作者：Shu-Chen Kao ; Chun-Yu Chen ; Shiu-Lan Wang ; Jaw-Ji Yang ; Wei-Chuan Hung ; Yi-Chen Chen ; Ning-Sheng Lai ; Hsin-Tzu Liu ; Hsien-lu Huang ; Hong-Chen Chen ; et al.
- 关键词：Phostensin ; KIAA1949 ; Protein phosphatase 1 ; Actin cytoskeleton
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 出版时间：11 May 2007
- 年：2007
- 卷：356
- 期：3
- 页码：594-598
- 全文大小：1271 K
文摘
We have identified a novel protein, protein phosphatase 1 F-actin cytoskeleton targeting subunit (phostensin). This protein is encoded by KIAA1949 and was found to associate with protein phosphatase 1 (PP1) in the yeast two-hybrid assay, co-immunoprecipitation, and GST pull-down assay. Northern blot analysis revealed that phostensin mRNA was predominantly distributed in leukocytes and spleen, and phostensin protein was present in crude extracts of human peripheral leukocytes. Immunofluorescence microscopic analysis revealed that the phostensin/PP1 complex was conspicuously localized with the actin cytoskeleton at the cell periphery in Madin–Darby canine kidney (MDCK) epithelial cells. Taken together, our data shows that phostensin targets PP1 to F-actin cytoskeleton. The phostensin/PP1 complex may play a vital role in modulation of actin rearrangements.