Purification and biological characterization of soluble, recombinant mouse IFN尾 expressed in insect cells

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• 作者：Sebastian A. Stifter ; Jodee A. Gould ; Niamh E. Mangan ; Hugh H. Reid ; Jamie Rossjohn ; Paul J. Hertzog ; Nicole A. de Weerd
• 关键词：Interferon beta ; Insect cell expression ; Soluble protein ; Natively folded ; Endotoxin-free ; Biologically active
• 刊名：Protein Expression and Purification
• 出版年：February, 2014
• 年：2014
• 卷：94
• 期：Complete
• 页码：7-14
• 全文大小：861 K

文摘

Interferon 尾 (IFN尾) is a member of the type I interferon family of cytokines widely recognised for their anti-viral, anti-proliferative and immunomodulatory properties. Recombinant, biologically active forms of this cytokine are used clinically for the treatment of multiple sclerosis and in laboratories to study the role of this cytokine in health and disease. Established methods for expression of IFN尾 utilise either bacterial systems from which the insoluble recombinant proteins must be refolded, or mammalian expression systems in which large volumes of cell culture are required for recovery of acceptable yields. Utilising the baculovirus expression system and Trichoplusia ni (Cabbage Looper) BTI-TN-5B1-4 cell line, we report a reproducible method for production and purification of milligram/litre quantities of biologically active murine IFN尾. Due to the design of our construct and the eukaryotic nature of insect cells, the resulting soluble protein is secreted allowing purification of the Histidine-tagged natively-folded protein from the culture supernatant. The IFN尾 purification method described is a two-step process employing immobilised metal-ion affinity chromatography (IMAC) and reverse-phase high performance liquid chromatography (RP-HPLC) that results in production of significantly more purified IFN尾 than any other reported eukaryotic-based expression system. Recombinant murine IFN尾 produced by this method was natively folded and demonstrated hallmark type I interferon biological effects including antiviral and anti-proliferative activities, and induced genes characteristic of IFN尾 activity in vivo. Recombinant IFN尾 also had specific activity levels exceeding that of the commercially available equivalent. Together, our findings provide a method for production of highly pure, biologically active murine IFN尾.