Lipid raft-dependent endocytosis negatively regulates responsiveness of J774 macrophage-like cells to LPS by down regulating the cell surface expression of LPS receptors
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- 作者：Szczepan Jó ; zefowski ; szjozefowski@poczta.onet.pl ; Małgorzata Śró ; ttek
- 关键词：CLIC ; clathrin-independent carrier ; EGF ; epidermal growth factor ; FCS ; fetal calf serum ; GEEC ; GPI-anchored-protein-enriched endosomal compartment ; GPI ; glycosylphosphatidylinositol ; GPI-AP ; GPI-anchored protein ; IFN ; interferon ; IL-6 ; interleukin-6 ; LPS ; lipopolysaccharide ; MβCD ; methyl-β-cyclodextrin ; MyD88 ; myeloid differentiation primary response gene 88 ; PBS ; phosphate-buffered saline ; PI-PLC ; phosphatidylinositol-specific phospholipase C ; RANTES ; regulated on activation ; normal T cell expr
- 刊名：Cellular Immunology
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：312
- 期：Complete
- 页码：42-50
- 全文大小：1477 K
- 卷排序：312
文摘
Clathrin-dependent endocytosis of TLR4 does not lead to its down-regulation. Lipid raft-dependent endocytosis reduces the cell surface level of LPS receptors. Disruption of lipid rafts does not block LPS-stimulated cytokine production. Inhibitors of CD14 signaling within lipid rafts do not inhibit responses to LPS. Lipid rafts may be sites of TLR4 and CD14 sorting for endocytosis.