- 作者：Yukio Tsushima ; Jae-Hwi Jang ; Moritz C. Wurnig ; Andreas Boss ; Kenji Suzuki ; Walter Weder ; Wolfgang Jungraithmayr
- 关键词：Mouse ; Lung transplantation ; Complications
- 刊名：Journal of Surgical Research
- 出版年：November, 2013
- 年：2013
- 卷：185
- 期：1
- 页码：426-432
- 全文大小：945 K
Background
Mouse lung transplantation has evolved into an established scientific model that is currently used by an increasing number of research groups. Acquiring this technique without previous microsurgical knowledge is considered very difficult. Disclosing all the intraoperative failures and mistakes during the model鈥檚 evolution will encourage all researchers who lack microsurgical skills that overcoming and eventually succeeding in this model is possible.Methods
Inbred (C57BL/6, BALB/c, SVG129) and CD1-outbred mice served as the transplant donors and recipients. The training procedure was performed by a surgeon not experienced in microsurgery, and arranged as follows: donor preparation until proof of functionality, graft implantation into deceased recipients, and graft implantation into surviving recipients until stable performance was achieved. The transplant鈥檚 viability was controlled using micro-computed tomography imaging.
Results
Donor preparation complications decreased from 43% after 1聽month to 0% after 2聽mo. The first functional donor was implanted at day 28, and the first successful implantation into a surviving recipient was performed at day 60 after six training recipients. Micro-computed tomography confirmed a ventilated and perfused graft. Intraoperative complications, mainly due to anastomosis failure, decreased from 58% after the first month to 15% at the latest assessment. The most recent implantation time was 75 卤 4.8聽min, and the transplantation success rate was 82% 卤 2.8%. A modified forceps considerably improved completion of the venous anastomosis.
Conclusions
Consistent success in the mouse lung transplantation model can be achieved even without pre-existing microsurgical skills. The surgery can be mastered within a reasonable period using a limited number of training animals. Procedure-related complications can be restricted to a minimum by applying key corrective steps at critical phases. This should encourage investigators without pre-expert knowledge in microsurgery to start to learn this research model.