Phospholamban is concentrated in the nuclear envelope of cardiomyocytes and involved in perinuclear/nuclear calcium handling

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• 作者：Adonis Z. Wu ; PhD^a ; ^b ; ¹ ; Dongzhu Xu ; MD ; PhD^a ; ^c ; Na Yang ; MD^a ; ^d ; Shien-Fong Lin ; PhD^a ; ^b ; Peng-Sheng Chen ; MD^a ; Steven E. Cala ; PhD^e ; Zhenhui Chen ; PhD^a ; ^{zhechen@iu.edu}
• 关键词：CM ; cardiomyocyte ; Fab ; the Fab fragment of the monoclonal anti-PLB antibody 2D12 ; IP3R ; inositol 1 ; 4 ; 5-trisphosphate receptor ; nCa2  ; + ;   ; perinuclear/nuclear Ca2  ; + ;   ; NE ; nuclear envelope ; NM ; perinuclear/nuclear membranes ; PLB ; phospholamban ; RyR2 ; ryanodine receptor ; SCW ; spontaneous Ca2  ; + wave ; SR ; sarcoplasmic reticulum ; SERCA2a ; isoform of Ca2  ; +-ATPase in cardiac SR
• 刊名：Journal of Molecular and Cellular Cardiology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：100
• 期：Complete
• 页码：1-8
• 全文大小：1918 K
• 卷排序：100

文摘

We tested if phospholamban (PLB) is in nuclear envelope (NE) of cardiomyocytes (CMs). Multiple PLB antibodies stained CM perinuclear/nuclear membranes in several species. Immunoblot/fluorescence assays showed high levels of PLB in NE of isolated CM nuclei. The Fab fragment of PLB antibody increased perinuclear/nuclear Ca uptake and release. PLB was localized in NE in high concentration and involved in CM nuclear Ca handling.