Emergence and dissemination of bla_OXA-23-carrying imipenem-resistant Acinetobacter sp in a regional hospital in Taiwan

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• 作者：Ming-Feng Lin^a ; Han-Yueh Kuo^b ; Hui-Wen Yeh^b ; Chih-Man Yang^b ; Chih-Hung Sung^c ; Chi-Chao Tu^a ; ^c ; Mei-Luan Huang^b ; Ming-Li Liou^c ; ^{d918229@gmail.com}
• 关键词：Acinetobacter sp ; blaOXA-23 gene ; blaOXA-51 gene ; ISAba1 ; Pulsed-field gel electrophoresis
• 刊名：Journal of Microbiology, Immunology and Infection
• 出版年：2011
• 出版时间：February 2011
• 年：2011
• 卷：44
• 期：1
• 页码：39-44
• 全文大小：264 K

文摘

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Background

The distribution and characterization of OXA-type carbapenemases in Acinetobacter sp in Taiwan has less been reported. The aim of the study was to investigate the molecular epidemiology and OXA-type carbapenemase genes in a regional hospital in Taiwan.

Methods

Imipenem-resistant Acinetobacter sp were collected between 2005 and 2007 in a regional hospital. Genotyping was performed by pulsed-field gel electrophoresis. OXA-type carbapenemase genes were determined by multiplex polymerase chain reaction (PCR) and gene sequencing.

Results

A total of 136 isolates were collected. Fifty-six pulsotypes were identified. None of the pulsotypes established predominance throughout the 3-year period. Multiplex PCR of bla_OXA genes showed that 99 % (135/136) of the Acinetobacter sp possessed bla_OXA51-like genes. The coexistences of bla_OXA51-like/bla_OXA-23-like and bla_OXA51-like/bla_OXA-24-like were detected in 19 % (26/136) and 1 % (2/136) of the isolates, respectively. Among bla_OXA-23-like gene-carrying isolates, two isolates (Pulsotypes 18 and 20) were found in 2006 and the remainder (n = 24), including Pulsotypes 27 (n = 18), 29 (n = 1), 52 (n = 3), and 53 (n = 2), were found in 2007. Sequencing performed on the 26 representative isolates confirmed the presence of the bla_OXA-23 carbapenemase gene. Analysis of the genetic content of bla_OXA-23 showed that these genes were presumably chromosomal and associated with the upstream-located insertion sequence ISAba1.

Conclusions

The emergence and imminent widespread of bla_OXA-23-carrying imipenem-resistant Acinetobacter sp appeared in Taiwan during the period from 2006 to 2007.