Development of a real-time PCR assay to control the illegal trade of meat from protected capercaillie species (Tetrao urogallus)

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• 作者：Mar&#xed ; a Rojas^a ; Isabel Gonz&#xe1 ; lez^a ; Miguel Á ; ngel Pavó ; n^a ; Nicolette Pegels^a ; Pablo E. Hern&#xe1 ; ndez^a ; Teresa Garc&#xed ; a^a ; Rosario Mart&#xed ; n ; ^a ; ^{rmartins@vet.ucm.es"" rel=""nofollow}
• 关键词：Capercaillie meat ; 12S rRNA gene ; 18S rRNA gene ; Real-time PCR
• 刊名：Forensic Science International
• 出版年：2011
• 出版时间：15 July 2011
• 年：2011
• 卷：210
• 期：1-3
• 页码：133-138
• 全文大小：297 K

文摘

A rapid and highly species-specific real-time polymerase chain reaction (PCR) assay has been developed for the detection of capercaillie DNA (Tetrao urogallus) in meat and meat mixtures. The method combines the use of capercaillie-specific primers, that amplify a 142 bp fragment of the mitochondrial 12S rRNA gene, and a positive control primer pair that amplifies a 141 bp fragment of the nuclear 18S rRNA gene from eukaryotic DNA. SYBR^® Green dye or TaqMan^® fluorogenic probes were used to monitor the amplification of the target genes. Results obtained with the use of TaqMan^® probes as detection platform increased the specificity of the real-time PCR assay in comparison with the results obtained using SYBR^® Green. The proposed real-time PCR assay represents a rapid and straightforward method for the accurate identification of capercaillie that could be used by law enforcement agencies as a tool for the control of poaching and illegal trade of meat from this protected species.