Prostaglandin E₂Induction of Binding Activity to CRE and AP-2 Elements in Human T Lymphocytes

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• 作者：Micali ; Ada ; Medici ; Nicola ; Sottile ; Antonino ; Venza ; Mario ; Venza ; Isabella ; Nigro ; Vincenzo ; et. al.
• 刊名：Cellular Immunology
• 出版年：1996
• 出版时间：November 25, 1996
• 年：1996
• 卷：174
• 期：1
• 页码：99-105
• 全文大小：2.69 M

文摘

Prostaglandins of the E series are immunomodulatory agents which exert inhibitory as well as stimulatory effects on a variety of immune responses. Since it is known that PGE₂is able to increase cAMP levels, we investigated whether it can affect gene expression through the activation of the transcription factors which bind enhancer elements in the promoter regions of cAMP-regulated genes. Using electrophoretic mobility shift assay, we demonstrated that a short treatment of human T lymphocytes with PGE₂induces specific binding activity to CRE and AP-2, but not AP-1, DNA elements. Since the okadaic acid, a potent protein phosphatase inhibitor, prolongs the induction of the binding activity, phosphorylation events are likely to occur. This activity seems to be due to increased cAMP levels because forskolin and IBMX mimic the effects of PGE₂. More interestingly, transfection experiments with CRE–CAT plasmide show that PGE₂activates the transcription of a CRE-containing promoter. These data support the positive role for PGE₂on some immune functions.