文摘
Hairy root cultures of Lobelia cardinalis clones Lc-A, induced by Agrobacterium rhizogenes ATCC 15834, and Lc-M, induced by A. rhizogenes MAFF 03-01724, were established. In hormone-free Murashige-Skoog liquid medium (50 ml medium per flask), both clones yielded high amounts of the polyacetylene glucosides, lobetyolin (18.7 mg per flask, Lc-M) and lobetyolinin (24.7 mg per flask, Lc-A), whose production level was the maximum in all cultures investigated.