Chiral separation of amino acids derivatised with fluorescein isothiocyanate by single isomer derivatives 3-monodeoxy-3-monoamino-¦Â- and ¦Ã-cyclodextrins: the effect of the cavity size

详细信息    查看全文

• 作者：Alessandro Giuffrida^a ; ^{agiuffri@unict.it} ; Rosario Caruso^a ; Marianna Messina^a ; Giuseppe Maccarrone^a ; Annalinda Contino^a ; Alejandro Cifuentes^b ; Vincenzo Cucinotta^a ; ^{ecucinotta@unict.it}
• 关键词：Capillary electrophoresis ; Cyclodextrin derivatives ; Molecular recognition ; Chiral separation ; Amino acids
• 刊名：Journal of Chromatography A
• 出版年：2012
• 出版时间：21 December, 2012
• 年：2012
• 卷：1269
• 期：Complete
• 页码：360-365
• 全文大小：512 K

文摘

Thirteen enantiomeric pairs of ¦Á-amino acids derivatised with fluorescein isothiocyanate (FITC-AAs) were separated in capillary electrophoresis (CE) using as chiral selectors the single isomer derivatives (SIDs) 3-monodeoxy-3-monoamino-¦Â- and ¦Ã-cyclodextrins. The chiral separation data obtained by these strictly homologous compounds, show different behaviours, allowing to hypothesise a possible structure of the obtained selector-analyte complexes, as well as highlighting the crucial role of the cavity size and the significant effects on the resolution obtained by small differences in the structural characteristics of these analytes.