Simultaneous extraction and clean-up of polychlorinated biphenyls and their metabolites from small tissue samples using pressurized liquid extraction

详细信息    查看全文

• 作者：Izabela Kania-Korwel ; Hongxia Zhao ; Karin Norstrom ; Xueshu Li ; Keri C. Hornbuckle ; Hans-Joachim Lehmler
• 关键词：Polychlorinated biphenyls (PCBs) ; Methylsulfonated biphenyls (MeSO₂-PCBs) ; Hydroxylated biphenyls (OH-PCBs) ; Pressurized liquid extraction (PLE)
• 刊名：Journal of Chromatography A
• 出版年：2008
• 出版时间：19 December 2008
• 年：2008
• 卷：1214
• 期：1-2
• 页码：37-46
• 全文大小：1451 K

文摘

A pressurized liquid extraction-based method for the simultaneous extraction and in situ clean-up of polychlorinated biphenyls (PCBs), hydroxylated (OH)-PCBs and methylsulfonyl (MeSO₂)-PCBs from small (<0.5 g) tissue samples was developed and validated. Extraction of a laboratory reference material with hexane–dichloromethane–methanol (48:43:9, v/v) and Florisil as fat retainer allowed an efficient recovery of PCBs (78–112 % ; RSD: 13–37 % ), OH-PCBs (46 ± 2 % ; RSD: 4 % ) and MeSO₂-PCBs (89 ± 21 % ; RSD: 24 % ). Comparable results were obtained with an established analysis method for PCBs, OH-PCBs and MeSO₂-PCBs.