Identification and quantification of flavonoids in Carica papaya leaf and peroxynitrite-scavenging activity

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• 作者：Agung Nugroho¹ ; ^{anugroho@unlam.ac.id} ; Hesty Heryani¹ ; Jae Sue Choi² ; Hee-Juhn Park³
• 关键词：Carica papaya ; Caricaceae ; Flavonoid ; HPLC quantification ; Peroxynitrite ; Kaempferol 3-(2G-rhamnosylrutinoside)
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：7
• 期：3
• 页码：208-213
• 全文大小：450 K
• 卷排序：7

文摘

To characterize the types, contents, and peroxynitrite-scavenging activities of flavonoids in the leaf of Carica papaya (C. papaya).MethodsChromatographic and spectroscopic techniques along with high performance liquid chromatography quantitative analysis and peroxynitrite-scavenging assay were performed to isolate and quantify flavonoid compounds in the flavonoid-rich fraction (BuOH fraction) derived from MeOH extract of C. papaya leaves and evaluate their peroxynitrite-scavenging activities.ResultsSeven flavonoids were isolated from the leaves of C. papaya, including quercetin 3-(2G-rhamnosylrutinoside), kaempferol 3-(2G-rhamnosylrutinoside), quercetin 3-rutinoside, myricetin 3-rhamnoside, kaempferol 3-rutinoside, quercetin, and kaempferol. All of the substances exhibited potent activities on peroxynitrite scavenging (IC50 ≤ 4.15 μmol/L), which were stronger than the positive control, l-penicillamine (6.90 μmol/L). The content of kaempferol 3-(2G-rhamnosylrutinoside) was significantly higher than other identified compounds (123.18 mg/g BuOH fraction and 7.23 mg/g MeOH extract).ConclusionsThe results of the present study demonstrate the potent antioxidant flavonoids of C. papaya leaf, with kaempferol 3-(2G-rhamnosylrutinoside) as the major one.