Fabrication of biodegradable textile scaffold based on hydrophobized hyaluronic acid

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• 作者：Vojtech Zapotocky^a ; ^b ; Martina Pospisilova^a ; ^{martinapospisilova@gmail.com} ; Katerina Janouchova^a ; Daniel Svadlak^a ; Jana Batova^a ; Jana Sogorkova^a ; Martin Cepa^a ; Jiri Betak^a ; Veronika Stepankova^a ; Romana Sulakova^a ; Jaromir Kulhanek^a ; Tomas Pitucha^a ; Jana Vranova^b ; Garry Duffy^c ; ^d ; ^e ; Vladimir Velebny^a
• 关键词：Palmitoyl-hyaluronan ; Wet-spinning ; Textile scaffold ; Fibrinogen ; Fibronectin ; Cell adhesion
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：903-909
• 全文大小：3034 K
• 卷排序：95

文摘

In this work, we report on the preparation of a novel biodegradable textile scaffold made of palmitoyl-hyaluronan (palHA). Monofilament fibres of palHA with a diameter of 120 μm were prepared by wet spinning. The wet-spun fibres were subsequently processed into a warp-knitted textile. To find a compromise between swelling in water and degradability of the final textile scaffold, a series of palHA derivatives with different degrees of substitution of the palmitoyl chain was synthesized. Freeze-drying not only provided shape fixation, but also speeded up scaffold degradation in vitro. Fibronectin, fibrinogen, laminin and collagen IV were physically adsorbed on the textile surface to enhance cell adhesion on the material. The highest amount of adsorbed cell-adhesive proteins was achieved with fibronectin (89%), followed by fibrinogen (81%). Finally, textiles modified with fibronectin or fibrinogen both supported the adhesion and proliferation of normal human fibroblasts in vitro, proving to be a useful cellular scaffold for tissue engineering.