Programmed Ribosomal Frameshifting Mediates Expression of the α-Carboxysome
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- 作者：Thawatchai Chaijarasphong1 ; Robert J. Nichols2 ; Kaitlyn E. Kortright2 ; Charlotte F. Nixon2 ; Poh K. Teng2 ; Luke M. Oltrogge2 ; David F. Savage1 ; 2 ; 3 ; savage@berkeley.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：CCM ; Carbon Dioxide Concentrating Mechanism ; PRF ; programmed ribosomal frameshifting ; RuBisCO ; ribulose-1 ; 5-bisphosphate carboxylase/oxygenase ; MW ; molecular weight ; EM ; electron microscopy ; MS ; mass spectrometry ; MS/MS ; tandem mass spectrometry ; MALDI-TOF ; matrix-assisted laser desorption/ionization&ndash ; time of flight ; GFP ; green fluorescent protein ; RFP ; red fluorescent protein ; IRES ; internal ribosome entry site ; WT ; wild type
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：16 January 2016
- 年：2016
- 卷：428
- 期：1
- 页码：153-164
- 全文大小：683 K
文摘
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The carboxysome protein CsoS2 is produced as two isoforms, each with an unknown role in the assembly of α-carboxysomes.
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We show that a − 1 programmed ribosomal frameshift is responsible for the production of CsoS2A.
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We express CsoS2A and CsoS2B individually for the first time.
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We conclude that only CsoS2B is necessary and sufficient for the structural formation of carboxysomes.