Absorbance and redox based approaches for measuring free heme and free hemoglobin in biological matrices
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- 作者：Joo-Yeun Ohg ; 1 ; Jennifer Hammb ; 1 ; Xin Xuc ; f ; Kristopher Genschmerc ; f ; Ming Zhongc ; i ; Jeffrey Lebensburgerb ; Marisa B. Marquesa ; Jeffrey D. Kerbyd ; h ; Jean-Francois Pittete ; Amit Gaggarc ; f ; h ; Rakesh P. Patela ; g ; rakeshpatel@uabmc.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Exosomes ; Microparticles ; Hemin
- 刊名：Redox Biology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：9
- 期：Complete
- 页码：167-177
- 全文大小：2356 K
文摘
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Heme and hemoglobin are redox active mediators of oxidative damage in multiple diseases.
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Limitations with current methods for free hemoglobin and heme measures are highlighted.
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New protocols for measuring free hemoglobin and heme simultaneously based on spectral deconvolution are provided.
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Improved accuracy for heme measures in stored red cells and levels higher than reported in Sickle cell disease is shown.