Enhancing yields of low and single copy number plasmid DNAs from Escherichia coli cells

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• 作者：Whitney N. Wood ; Kyle D. Smith ; Jennifer A. Ream ; L. Kevin Lewis ; ^{LL18@txstate.edu}
• 关键词：Plasmid ; Copy number ; DNA purification ; Miniprep ; TB ; LB
• 刊名：Journal of Microbiological Methods
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：133
• 期：Complete
• 页码：46-51
• 全文大小：658 K
• 卷排序：133

文摘

TB broth produced the greatest yields of low copy number plasmid DNA from minipreps. Yeast extract, not glycerol carbon source, was key to superior results seen with TB. Increasing yeast extract in the most commonly used broth, LB, made it equal to TB. Adding more ampicillin to enhance selection for plasmids did not improve DNA yields.