Preparation and characterization of Chinese yam polysaccharide PLGA nanoparticles and their immunological activity

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• 作者：Li Luo^a ; Sisi Zheng^a ; Yifan Huang^b ; Tao Qin^b ; Jie Xing^a ; Yale Niu^a ; Ruonan Bo^a ; Zhenguang Liu^a ; Yee Huang^a ; Yuanliang Hu^a ; Jiaguo Liu^a ; Yi Wu^a ; Deyun Wang^a ; ^b ; ^{dywang@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：CYP ; Chinese yam polysaccharide ; PLGA ; poly(D ; L-lactic-co-glycolic acid) ; CYPP ; Chinese yam polysaccharide PLGA nanoparticles ; BP ; blank PLGA nanoparticles ; NP ; nanoparticle ; PE ; primary emulsion ; RSM ; response surface methodology ; LPS ; lipopolysaccharide ; PHA ; Phytohemagglutinin ; MTT ; 3-(4 ; 5-Dimethylthiazol-2-yl)-2 ; 5-diphenyltetrazolium bromide ; PBS ; phosphate buffered saline ; EE ; Entrapment efficiency ; BBD ; Box-Behnken design ; ANOVA ; analysis of variance ; PDI ; polydispersity index
• 刊名：International Journal of Pharmaceutics
• 出版年：2016
• 出版时间：10 September 2016
• 年：2016
• 卷：511
• 期：1
• 页码：140-150
• 全文大小：2066 K

文摘

This paper first provides that Chinese yam polysaccharide (CYP) is encapsulated by PLGA using a double emulsion solvent evaporation method and aims to screen the optimal preparation of CYP-PLGA nanoparticles (CYPP) using response surface methodology (RSM). The volume ratio of the internal water phase to the organic phase (W₁:O), the volume ratio of the primary emulsion to the external water phase (PE:W₂) and the concentration of Poloxamer 188 (F68) are deemed key variables for the encapsulation efficiency of CYPP. The results demonstrated that the data were accurately fitted into the RSM model. According to the RSM, the optimal scheme was a volume ratio of W₁:O of 1:9, a volume ratio of PE: W₂ of 1:10 and a concentration of F68 (W/V) of 0.7%. TEM and SEM images demonstrated that the nanoparticles had a spherical shape and smooth surface. The CYP and CYPP in vitro release studies demonstrated that the CYPP showed a release rate 53.41% lower than the release rate of CYP after 48 h. The result of pro-proliferation and flow cytometry emerged that the CYPP were more effective compared with the free CYP and blank PLGA nanoparticles in promoting lymphocyte proliferation and triggering the transformation of T lymphocytes into Th cells.