Reversible immobilization of laccase onto metal-ion-chelated magnetic microspheres for bisphenol A removal
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- 作者：Jiahong Lin ; Yingju Liu ; Shi Chen ; chens1968@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Xueyi Le ; Xiaohua Zhou ; Zhiyong Zhao ; Yiyi Ou ; Jianhua Yang
- 关键词：MCMS ; magnetic microspheres ; BPA ; bisphenol A ; CS ; chitosan ; ABTS ; 2 ; 2&prime ; -azino-bis-(3-ethyl-benzothiazoline-6-sulphonic acid) ; GA ; glutaraldehyde ; PEG ; polyethylene glycol ; EDTA ; ethylene diamine tetraacetic acid ; SEM ; scanning electron microscopy ; TEM ; transmission electron microscopy ; FT-IRf ; ourier transform-infrared spectroscopy ; VSM ; vibrating sample magnetometer ; HPLC ; high-performance liquid chromatography
- 刊名：International Journal of Biological Macromolecules
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：84
- 期：Complete
- 页码：189-199
- 全文大小：2780 K
文摘
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In this study, laccase was reversibly immobilized on Cu(ΙΙ) and Mn(ΙΙ) chelated magnetic microspheres and successfully applied for the removal of bisphenol A (BPA) from water.
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The laccase adsorption capacities of the magnetic microspheres did not change after five successive adsorption–desorption cycles.
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The immobilized laccase exhibited high removal efficiency of BPA under the combined actions of biodegradation and adsorption.
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In comparison with free laccase, the thermal and storage stabilities of the immobilized laccase were significantly improved.