Highly sensitive turn-on fluorescence detection of thrombomodulin based on fluorescence resonance energy transfer

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• 作者：Liyan Kong^a ; Jiaming Zhu^a ; Wen Wang^a ; Lehe Jin^b ; Yanjiao Fu^b ; Bohui Duan^b ; Liang Tan^a ; ^b ; ^{liangtan@hunnu.edu.cn}
• 关键词：Thrombomodulin ; Immunoreaction ; Acridine orange ; Gold nanocomposites ; Fluorescence resonance energy transfer
• 刊名：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：173
• 期：Complete
• 页码：675-680
• 全文大小：936 K
• 卷排序：173

文摘

The fluorescence of AO was quenched by gold nanocomposites due to FRET. The binding of TM on BSA-AuNPs-Ab led to a decreased FRET efficiency. A highly sensitive method for the quantitative detection of TM was developed. The release of sTM by the injured HUVEC-C cells was investigated.