Metabolomic analysis of glycerophospholipid signatures of inflammation treated with non-steroidal anti-inflammatory drugs-induced-RAW264.7 cells using 1H NMR and U-HPLC/Q-TOF-MS
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- 作者：Xia Wu ; Han Cao ; Lifang Zhao ; Jianao Song ; Yuqi She ; Yifan Feng ; yffeng@139.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：1H NMR ; U-HPLC/Q-TOF-MS ; Glycerolphospholipids ; RAW264.7 cells ; Chemometrics ; Biomarkers
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 August 2016
- 年：2016
- 卷：1028
- 期：Complete
- 页码：199-215
- 全文大小：4447 K
文摘
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1H NMR and U-HPLC/Q-TOF-MS were applied to analyze changes of GPLs in RAW264.7 cells.
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1H NMR could be used as a new screening tool for the metabolic profiling of GPLs.
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19 GPLs were firstly identified compared with our previous study by U-HPLC/Q-TOF-MS.
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Ten potential biomarkers were proved by U-HPLC/Q-TOF-MS coupled to chemometrics.
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PC (16:0/18:1) and (18:0/18:1) may be important biomarkers due to consistent change.