Optimization of microwave-assisted FeCl₃ pretreatment conditions of rice straw and utilization of Trichoderma viride and Bacillus pumilus for production of reducing sugars

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• 作者：Jiliang L&#xfc ; ^a ; ^{ljl_82919@163.com"" rel=""nofollow} ; Peijiang Zhou ; ^a ; ^{lvkevin@yahoo.cn"" rel=""nofollow}
• 关键词：Rice straw ; Microwave-assisted FeCl₃ pretreatment ; RSM optimization ; Trichoderma viride ; Bacillus pumilus
• 刊名：Bioresource Technology
• 出版年：2011
• 出版时间：July 2011
• 年：2011
• 卷：102
• 期：13
• 页码：6966-6971
• 全文大小：855 K

文摘

In this study, Box-Behnken design (BBD) and response surface methodology (RSM) were used to optimize microwave-assisted FeCl₃ pretreatment conditions of rice straw with respect to FeCl₃ concentration, microwave intensity, irradiation time and substrate concentration. When rice straw was pretreated at the optimal conditions of FeCl₃ concentration, 0.14 mol/L; microwave intensity, 160 °C; irradiation time, 19 min; substrate concentration, 109 g/L; and inoculated with Trichoderma viride and Bacillus pumilus, the production of reducing sugars was 6.62 g/L. This yield was 2.9 times higher than that obtained with untreated rice straw. The microorganisms degraded 37.8 % of pretreated rice straw after 72 h. The structural characteristic analyses suggest that microwave-assisted FeCl₃ pretreatment damaged the silicified waxy surface of rice straw, disrupted almost all the ether linkages between lignin and carbohydrates, and removed lignin.