Simultaneous determination of nine bioactive compounds in Yijin-tang via high-performance liquid chromatography and liquid chromatography-electrospray ionization-mass spectrometry

详细信息    查看全文

• 作者：Hye Jin Yang ; Nam-Hui Yim ; Kwang Jin Lee ; Min Jung Gu ; Bohyoung Lee ; Youn-Hwan Hwang ; ^{hyhhwang@kiom.re.kr" class="auth_mail" title="E-mail the corresponding author} ; Jin Yeul Ma ; ^{jyma@kiom.re.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：HPLC-DAD ; LC/MS ; simultaneous determination ; validation ; Yijin-tang
• 刊名：Integrative Medicine Research
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：5
• 期：2
• 页码：140-150
• 全文大小：2080 K

文摘

Yijin-tang (YJ) has been used traditionally for the treatment of cardiovascular conditions, nausea, vomiting, gastroduodenal ulcers, and chronic gastritis. In this study, a simple and sensitive high-performance liquid chromatography (HPLC) method was developed for the quantitation of nine bioactive compounds in YJ: homogentisic acid, liquiritin, naringin, hesperidin, neohesperidin, liquiritigenin, glycyrrhizin, 6-gingerol, and pachymic acid.

Methods

Chromatographic separation of the analytes was achieved on an RS Tech C₁₈ column (4.6 mm × 250 mm, 5 μm) using a mobile phase composed of water containing 0.1% (v/v) trifluoroacetic acid (TFA) and acetonitrile with a gradient elution at a flow rate of 1.0 mL/min.

Results

Calibration curves for all analytes showed good linearity (R² ≥ 0.9995). Lower limits of detection and lower limits of quantification were in the ranges of 0.03–0.17 μg/mL and 0.09–0.43 μg/mL, respectively. Relative standard deviations (RSDs; %) for intra- and interday assays were < 3%. The recovery of components ranged from 98.09% to 103.78%, with RSDs (%) values ranging from 0.10% to 2.59%.

Conclusion

This validated HPLC method was applied to qualitative and quantitative analyses of nine bioactive compounds in YJ and fermented YJ, and may be a useful tool for the quality control of YJ.