Proteomic analysis of Eimeria acervulina sporozoite proteins interaction with duodenal epithelial cells by shotgun LC-MS/MS
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- 作者：ZhenChao Zhang ; Shuai Wang ; JingWei Huang ; LianRui Liu ; MingMin Lu ; MengHui Li ; YuXia Sui ; LiXin Xu ; RuoFeng Yan ; XiaoKai Song ; XiangRui Li ; lixiangrui@njau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：E. acervulina ; Sporozoite ; Chicken ; Duodenal epithelial cells
- 刊名：Molecular & Biochemical Parasitology
- 出版年：2015
- 出版时间：August 2015
- 年：2015
- 卷：202
- 期：2
- 页码：29-33
- 全文大小：827 K
文摘
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The proteins of chicken duodenal epithelial cells cultured with soluble proteins of E. acervulina sporozoite were analyzed by Western blot.
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Total 85 proteins of E. acervulina were identified from Eimeria protein database by using shotgun LC–MS/MS and MASCOT engine searches.
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Functional analysis of E. acervulina proteins binding to chicken duodenal epithelial cells was performed.
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Several novel proteins were related to the process of host cell invasion or modulation of the host celluar functions.