Comparative vesicle proteomics reveals selective regulation of protein expression in chestnut blight fungus by a hypovirus

详细信息    查看全文

• 作者：Jinzi Wang ; Fangzhen Wang ; Youjun Feng¹ ; Ke Mi ; Qi Chen ; Jinjie Shang ; Baoshan Chen ; ^{chenyaoj@gxu.edu.cn}
• 关键词：Vesicle proteome ; Regulation ; Hypovirus ; Chestnut blight fungus
• 刊名：Journal of Proteomics
• 出版年：2013
• 出版时间：14 January, 2013
• 年：2013
• 卷：78
• 期：Complete
• 页码：221-230
• 全文大小：967 K

文摘

The chestnut blight fungus (Cryphonectria parasitica) and hypovirus constitute a model system to study fungal pathogenesis and mycovirus-host interaction. Knowledge in this field has been gained largely from investigations at gene transcription level so far. Here we report a systematic analysis of the vesicle proteins of the host fungus with/without hypovirus infection. Thirty-three differentially expressed protein spots were identified in the purified vesicle protein samples by two-dimensional electrophoresis and mass spectrometry. Down-regulated proteins were mostly cargo proteins involved in primary metabolism and energy generation and up-regulated proteins were mostly vesicle associated proteins and ABC transporter. A virus-encoded protein p48 was found to have four forms with different molecular mass in vesicles from the virus-infected strain. While a few of the randomly selected differentially expressed proteins were in accordance with their transcription profiles, majority were not in agreement with their mRNA accumulation patterns, suggesting that an extensive post-transcriptional regulation may have occurred in the host fungus upon a hypovirus infection.