Molecular cloning, characterization and mRNA expression of six peroxiredoxins from Black carp Mylopharyngodon piceus in response to lipopolysaccharide challenge or dietary carbohydrate
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- 作者：Chenglong Wu ; wuchenglong007@163.com" class="auth_mail" title="E-mail the corresponding author ; Jun'e Gao ; Fang Cao ; Zhibin Lu ; Lian Chen ; Jinyun Ye
- 关键词：Peroxiredoxin ; Mylopharyngodon piceus ; mRNA expression ; Lipopolysaccharide challenge ; Dietary carbohydrate
- 刊名：Fish & Shellfish Immunology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：50
- 期：Complete
- 页码：210-222
- 全文大小：2087 K
文摘
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Six peroxiredoxins (Prxs) of black carp (MpPrx) have been cloned and characterized.
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The phylogenetic analysis evidenced three major subclasses corresponding to one-Cys-Prx (MpPrx6), typical two-Cys-Prx (MpPrx1-4) and atypical 2-Cys-Prx (MpPrx5).
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LPS stimulation could induce up-expression of six MpPrxs mRNA in the liver of Black carp.
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Different levels of dietary carbohydrate could regulate the expression patterns of six MpPrxs.
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Higher levels of MpPrxs might play important roles in removing ROS and maintaining redox homeostasis in Black carp.