Fast, robust and high-resolution glycosylation profiling of intact monoclonal IgG antibodies using nanoLC-chip-QTOF
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- 作者：Joannes F.M. Jacobsa ; Ron A. Weversb ; Dirk J. Lefeberb ; c ; Monique van Scherpenzeelb ; monique.vanscherpenzeel@radboudumc.nl" class="auth_mail" title="E-mail the corresponding author
- 关键词：Monoclonal Gammopathy ; Therapeutic monoclonal antibodies ; Cryoglobulin ; QTOF high resolution mass spectrometry ; Intact protein analysis
- 刊名：Clinica Chimica Acta
- 出版年：2016
- 出版时间：1 October 2016
- 年：2016
- 卷：461
- 期：Complete
- 页码：90-97
- 全文大小：1259 K
文摘
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Quantitative glycosylation profiling of intact immunoglobulin
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Application on therapeutic antibodies and multiple myeloma patient sera
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Quantitative detection of hypoglycosylation, i.e. the lack of whole glycans
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Fast and robust high-resolution mass spectrometry
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No digestion steps involved