Kinetic metabolic modelling for the control of plant cells cytoplasmic phosphate

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• 作者：Mathieu Cloutier ; Jingkui Chen ; Frithjof Tatge ; Virginie McMurray-Beaulieu ; Michel Perrier ; Mario Jolicoeur
• 关键词：Dynamic metabolic modelling ; Metabolic regulation ; Phosphate metabolism ; Plant cell culture
• 刊名：Journal of Theoretical Biology
• 出版年：2009
• 出版时间：7 July 2009
• 年：2009
• 卷：259
• 期：1
• 页码：118-131
• 全文大小：1012 K

文摘

A previously developed kinetic metabolic model for plant metabolism was used in a context of identification and control of intracellular phosphate (Pi) dynamics. Experimental data from batch flask cultures of Eschscholtiza californica cells was used to calibrate the model parameters for the slow dynamics (growth, nutrition, anabolic pathways, etc.). Perturbation experiments were performed using a perfusion small-scale bioreactor monitored by in vivo ³¹P NMR. Parameter identification for Pi metabolism was done by measuring the cells dynamic response to different inputs for extracellular Pi (two pulse-response experiments and a step-response experiment). The calibrated model can describe Pi translocation between the cellular pools (vacuole and cytoplasm). The effect of intracellular Pi management on ATP/ADP and phosphomonoesters concentrations is also described by the model. The calibrated model is then used to develop a control strategy on the cytoplasmic Pi pool. From the identification of the systems dynamics, a proportional-integral controller was designed and tuned. The closed-loop control was implemented in the small-scale NMR bioreactor and experimental results were in accordance with model predictions. Thus, the calibrated model is able to predict cellular behaviour for phosphate metabolism and it was demonstrated that it is possible to control the intracellular level of cytoplasmic Pi in plant cells.