A user-friendly, highly sensitive assay to detect the IFN-γ secretion by T cells
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- 作者：Paul L.A.M. Corstjens ; Michel Zuiderwijk ; Hans J. Tanke ; Jolien J. van der Ploeg-van Schip ; Tom H.M. Ottenhoff ; Annemiek Geluk
- 关键词：Cell mediated immunity ; IFN-γ ; Interferon ; Lateral flow ; Leprosy ; Mycobacteria ; UCP ; Up-converting phosphor ; UPT
- 刊名：Clinical Biochemistry
- 出版年：2008
- 出版时间：April 2008
- 年：2008
- 卷：41
- 期：6
- 页码：440-444
- 全文大小：347 K
文摘
Development of a user-friendly test alternative to ELISA-based assays to detect IFN-γ by in vitro cultured peripheral blood mononuclear cells (PBMC) stimulated with pathogen-derived antigens.