GAPTrap: A Simple Expression System for Pluripotent Stem Cells and Their Derivatives

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• 作者：Tim Kao¹ ; ² ; Tanya Labonne¹ ; Jonathan C. Niclis⁴ ; Ritu Chaurasia² ; Zerina Lokmic¹ ; Elizabeth Qian¹ ; ² ; Freya F. Bruveris¹ ; ³ ; Sara E. Howden¹ ; ² ; Ali Motazedian¹ ; ² ; Jacqueline V. Schiesser³ ; ⁸ ; Magdaline Costa⁹ ; Koula Sourris¹ ; Elizabeth Ng¹ ; David Anderson¹ ; Antonietta Giudice³ ; Peter Farlie¹ ; ² ; Michael Cheung¹ ; ² ; ⁷ ; Shireen R. Lamande¹ ; ² ; Anthony J. Penington¹ ; ² ; Clare L. Parish⁴ ; Lachlan H. Thomson⁴ ; Arash Rafii⁵ ; ⁶ ; David A. Elliott¹ ; ¹⁰ ; Andrew G. Elefanty¹ ; ² ; ³ ; ^{andrew.elefanty@mcri.edu.au" class="auth_mail" title="E-mail the corresponding author} ; Edouard G. Stanley¹ ; ² ; ³ ; ^{ed.stanley@mcri.edu.au" class="auth_mail" title="E-mail the corresponding author}
• 刊名：Stem Cell Reports
• 出版年：2016
• 出版时间：13 September 2016
• 年：2016
• 卷：7
• 期：3
• 页码：518-526
• 全文大小：2258 K

文摘

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d="p0010">GAPTrap vector system targets transgenes to the ubiquitously expressed GAPDH locus

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d="p0015">Targeting transgenes to the GAPDH locus yields reliable transgene expression

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d="p0020">Transgenes at this locus are robustly expressed in differentiated cells

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d="p0025">Generation of GAPTrap targeted human PSC lines is simple and efficient

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