Effects of gallic acid on delta - aminolevulinic dehydratase activity and in the biochemical, histological and oxidative stress parameters in the liver and kidney of diabetic rats

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• 作者：Lizielle Souza de Oliveira^a ; Gustavo Roberto Thomé ; ^a ; Thauan Faccin Lopes^a ; Karine Paula Reichert^a ; Juliana Sorraila de Oliveira^a ; Aline da Silva Pereira^a ; Jucimara Baldissareli^a ; Cristina da Costa Krewer^b ; Vera Maria Morsch^a ; Maria Rosa Chitolina Schetinger^a ; Roselia Maria Spanevello^c ; ^{rspanevello@gmail.com}
• 关键词：ALT ; alanine aminotransferase ; AST ; aspartate aminotransferase ; CAT ; catalase ; δ-ALA ; δ-aminolevulinic acid ; δ-ALA-D ; delta-aminolevulinic acid dehydratase ; DCF ; dichlorofluorescein ; DCFH-DA ; dichloro-dihydro-fluorescein diacetate ; DM ; diabetes melitus ; DNPH ; dinitrophenyl hydrazine ; GA ; gallic acid ; GST ; glutathione S-transferase ; HDL ; high density lipoprotein ; LDL ; low density lipoprotein ; PBG ; porphobilinogen ; ROS ; reactive oxygen species ; RS ; reactive species ; SD ; standard deviation ; SDS ; so
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：84
• 期：Complete
• 页码：1291-1299
• 全文大小：1361 K
• 卷排序：84

文摘

Diabetes mellitus (DM) is characterised by hyperglycaemia associated with the increase of oxidative stress. Gallic acid has potent antioxidant properties. The aim of this study was to evaluate the effect of gallic acid on the biochemical, histological and oxidative stress parameters in the liver and kidney of diabetic rats. Male rats were divided in groups: control, gallic acid, diabetic and diabetic plus gallic acid. DM was induced in the animals by intraperitoneal injection of streptozotocin (65 mg/kg). Gallic acid (30 mg/kg) was administered orally for 21 days. Our results showed an increase in reactive species levels and lipid peroxidation, and a decrease in activity of the enzymes superoxide dismutase and delta-aminolevulinic acid dehydratase in the liver and kidney of the diabetic animals (P < 0.05). Gallic acid treatment showed protective effects in these parameters evaluated, and also prevented a decrease in the activity of catalase and glutathione S-transferase, and vitamin C levels in the liver of diabetic rats. In addition, gallic acid reduced the number of nuclei and increased the area of the core in hepatic tissue, and increased the glomerular area in renal tissue. These results indicate that gallic acid can protect against oxidative stress-induced damage in the diabetic state.