Proton Dipolar Recoupling in Resin-Bound Peptides under High-Resolution Magic Angle Spinning

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• 作者：Raya ; Jé ; sus ; Bianco ; Alberto ; Furrer ; Julien ; Briand ; Jean-Paul ; Piotto ; Martial ; Elbayed ; Karim
• 关键词：HRMAS ; dipolar recoupling ; rotational resonance ; radiofrequency-driven dipolar recoupling ; NOE ; peptides
• 刊名：Journal of Magnetic Resonance
• 出版年：2002
• 出版时间：July, 2002
• 年：2002
• 卷：157
• 期：1
• 页码：43-51
• 全文大小：594 K

文摘

Rotational resonance and radiofrequency-driven dipolar recoupling (RFDR) experiments have been used to recover the weak proton dipolar interaction present in peptides bound to swollen resins spun at the magic angle. The intensity of the correlation peaks obtained using these sequences is shown to be significantly stronger than the one obtained using the classical NOESY experiment. In addition, it is found that during the relatively long mixing times required to transfer magnetization in such soft materials, the RFDR sequence also achieves magnetization transfer via the scalar J-coupling.