文摘
Administration of dihomo-γ-linolenic acid is useful for atopic dermatitis and atherosclerosis in mice; however, the metabolites of dihomo-γ-linolenic acid have been little studied. We employed a method which enabled simultaneous analysis of nine prostaglandins using liquid chromatographyx2013;tandem mass spectrometry, and determined the concentrations of prostaglandins in the supernatants of cultures of mouse peritoneal macrophages stimulated with lipopolysaccharide after pre-incubation with dihomo-γ-linolenic acid, arachidonic acid, or eicosapentaenoic acid. Accumulated prostaglandin concentrations from mouse macrophages with dihomo-γ-linolenic acid uptake increased in a dihomo-γ-linolenic acid concentration-dependent fashion. These increases were mainly due to prostaglandin D1 and prostaglandin E1. The order of accumulated prostaglandin concentrations was dihomo-γ-linolenic acid>arachidonic acid>eicosapentaenoic acid in supernatants with the same concentration of polyunsaturated fatty acid. Since mouse macrophages can clearly produce series-1 prostaglandins, they must be formed in vivo. These findings suggest that the effects of dihomo-γ-linolenic acid on diseases may be due to series-1 prostaglandins.