On the use of peroxy-caged luciferin (PCL-1) probe for bioluminescent detection of inflammatory oxidants in vitro and in vivo - Identification of reaction intermediates and oxidant-specific minor products
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- 作者：Jacek Zielonkaa ; jzielonk@mcw.edu" class="auth_mail" title="E-mail the corresponding author ; Radosław Podsiadłyb ; radoslaw.podsiadly@p.lodz.pl" class="auth_mail" title="E-mail the corresponding author ; Monika Zielonkaa ; mzielonka@mcw.edu" class="auth_mail" title="E-mail the corresponding author ; Micael Hardyc ; micael.hardy@univ-provence.fr" class="auth_mail" title="E-mail the corresponding author ; Balaraman Kalyanaramana ; balarama@mcw.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：AA-OOH ; amino acid hydroperoxide ; DIPPMPO ; diisopropoxyphosphoryl-5-methyl-1-pyrroline N-oxide ; DPI ; diphenyleneiodonium ; HO-Bz-OH ; 4-hydroxybenzyl alcohol ; L-NAME ; L-NG-Nitroarginine methyl ester ; LOO&bull ; lipid peroxy radicals ; LOOH ; lipid hydroperoxide ; Luc-Cl ; chloroluciferin ; MNP ; 2-methyl-2-nitroso propane ; MRM ; multiple reaction monitoring ; PCL-1 ; peroxy-caged luciferin ; Pr-OOH ; protein hydroperoxide ; QM ; para-quinone methide ; RNS ; reactive nitrogen species ; ROS ; reactive oxygen species
- 刊名：Free Radical Biology and Medicine
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：99
- 期：Complete
- 页码：32-42
- 全文大小：2543 K
文摘
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PCL-1 reacts with H2O2, HOCl, and ONOO− to form luciferin as the major product.
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ONOO− oxidizes PCL-1 nearly a million times more rapidly than does H2O2.
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Nitrobenzylated luciferin is a minor, ONOO−-specific product of PCL-1.
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PCL-1 reaction with excess HOCl generates a chlorinated luciferin.
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Minor products of PCL-1 may serve as specific markers for ONOO− and HOCl.