Complex transcriptional regulation of citrate metabolism in Clostridium perfringens

详细信息    查看全文

• 作者：Yonghui Yuan ; Kaori Ohtani ; Satoko Yoshizawa ; Tohru Shimizu ; ^{tshimizu@med.kanazawa-u.ac.jp}
• 关键词：Pathogenesis ; Two-component system ; Energy metabolism
• 刊名：Anaerobe
• 出版年：2012
• 出版时间：February 2012
• 年：2012
• 卷：18
• 期：1
• 页码：48-54
• 全文大小：557 K

文摘

A Gram-positive, spore-forming bacterium, Clostridium perfringens, possesses genes for citrate metabolism, which might play an important role in the utilization of citrate as a sole carbon source. In this study, we identified a chromosomal citCDEFX-mae-citS operon in C. perfringens strain 13, which is transcribed on three mRNAs of different sizes. Expression of the cit operon was significantly induced when 5?mM extracellular citrate was added to the growth medium. Most interestingly, three regulatory systems were found to be involved in the regulation of the expression of cit genes: 1) the two upstream divergent genes citG and citI; 2) two different two-component regulatory systems, CitA/CitB (TCS6 consisted of CPE0531/CPE0532) and TCS5 (CPE0518/CPE0519); and 3) the global two-component VirR/VirS-VR-RNA regulatory system known to regulate various genes for toxins and degradative enzymes. Our results suggest that in C. perfringens the citrate metabolism might be strictly controlled by a complex regulatory system.