Paperback. 190 Seiten. € 16,95. sFr 28,00Maria-Anna Schoppmeyer, Anatomie und Physiologie — Prüfungswissen für Pflegeberufe (2. Auflage), Urban & Fischer Verlag, Basel (2002) I

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• 作者：Kathrin Kalies
• 关键词：Histochemistry ; Glycoconjugates ; Oocyte-Zona pellucida ; Wild ungulates
• 刊名：Annals of Anatomy - Anatomischer Anzeiger
• 出版年：2003
• 出版时间：April 2003
• 年：2003
• 卷：185
• 期：2
• 页码：116
• 全文大小：76 K

文摘

The aim of the present study was to examine the glycoconjugate modifications occurring in the zona pellucida during oocyte growth in fallow, red and roe deer using a battery of lectins combined with sialidase digestion and chemical treatments. This histochemical approach allowed us to sequence the oligosaccharidic side chains of the zona pellucida glycoproteins in these wild ungulates. The most effective lectins in the zona pellucida of these species were SBA, PNA, RCA-I GSA-IB4, and WGA, indicating the presence of β-D-N-Acetylgalactosamine, β-D-Galactose, α-D-Galactose and N-Acetylglucosamine residues. Additionally, sialic acid moieties were demonstrated. We also observed differences in the glycosidic residue content and in their spatial distribution, depending on the species and stage of follicle development.