Facile preparation of highly crystalline lamellae of (1 鈫?#xa0;3)-尾-d-glucan using an extract of Euglena gracilis

详细信息    查看全文

• 作者：Yu Ogawa ; Kazuhiro Noda ; Satoshi Kimura ; Motomitsu Kitaoka ; Masahisa Wada
• 关键词：(1  ; &rarr ;   ; 3)-尾-d-Glucan ; In vitro synthesis ; Laminaribiose phosphorylase
• 刊名：International Journal of Biological Macromolecules
• 出版年：March, 2014
• 年：2014
• 卷：64
• 期：Complete
• 页码：415-419
• 全文大小：1416 K

文摘

In vitro synthesis of (1 鈫?#xA0;3)-尾-d-glucan was performed using laminaribiose phosphorylase obtained by an extraction of Euglena gracilis with sucrose phosphorylase. The synthetic product was a linear (1 鈫?#xA0;3)-尾-d-glucan with a narrow distribution of degree of polymerization (DP) centered on DP = 30. X-ray diffraction and electron microscopy revealed that the glucan molecules obtained were self-organized as highly crystalline hexagonal lamellae. This synthetic product has quite high structural homogeneity at every level from primary to higher-order structure, which is a great advantage for the detailed analyses of physiological functions of (1 鈫?#xA0;3)-尾-d-glucan.