- 作者：Kristy A. Warner ; April Adams ; Lisiane Bernardi ; Carolina Nor ; Kelsey A. Finkel ; Zhaocheng Zhang ; Scott A. McLean ; Joseph Helman ; Gregory T. Wolf ; Vasu Divi ; Lurdes Queimado ; Frederic J. Kaye ; Rogerio M. Castilho ; Jacques E. N?r
- 关键词：Mouse models ; Salivary gland cancer ; Xenograft ; Oral cancer ; Crtc1&ndash ; Maml2 ; Tumor recurrence ; Metastasis
- 刊名：Oral Oncology
- 出版年：2013
- 出版时间：November, 2013
- 年：2013
- 卷：49
- 期：11
- 页码：1059-1066
- 全文大小：3909 K
Summary
The long-term outcome of patients with mucoepidermoid carcinoma is poor. Limited availability of cell lines and lack of xenograft models is considered a major barrier to improved mechanistic understanding of this disease and development of effective therapies.Objective
To generate and characterize human mucoepidermoid carcinoma cell lines and xenograft models suitable for mechanistic and translational studies.
Methods
Five human mucoepidermoid carcinoma specimens were available for generation of cell lines. Cell line tumorigenic potential was assessed by transplantation and serial in vivo passaging in immunodeficient mice, and cell line authenticity verified by short tandem repeat (STR) profiling.
Results
A unique pair of mucoepidermoid carcinoma cell lines was established from a local recurrence (UM-HMC-3A) and from the metastatic lymph node (UM-HMC-3B) of the same patient, 4 years after surgical removal of the primary tumor. These cell lines retained epithelial-like morphology through 100 passages in vitro, contain the Crtc1-Maml2 fusion oncogene (characteristic of mucoepidermoid carcinomas), and express the prototypic target of this fusion (NR4A2). Both cell lines generated xenograft tumors when transplanted into immunodeficient mice. Notably, the xenografts exhibited histological features and Periodic Acid Schiff (PAS) staining patterns that closely resembled those found in human tumors. STR profiling confirmed the origin and authenticity of these cell lines.
Conclusion
These data demonstrate the generation and characterization of a pair of tumorigenic salivary mucoepidermoid carcinoma cell lines representative of recurrence and lymph node metastasis. Such models are useful for mechanistic and translational studies that might contribute to the discovery of new therapies for mucoepidermoid carcinoma.