Protection of mice from Shiga toxin-2 toxemia by mucosal vaccine of Shiga toxin 2B-His with Escherichia coli enterotoxin

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• 作者：Takao Tsuji ; Takeshi Shimizu ; Keiko Sasaki ; Yoshiyasu Shimizu ; Kentaro Tsukamoto ; Hideyuki Arimitsu ; Sadayuki Ochi ; Satoshi Sugiyama ; Koki Taniguchi ; Paola Neri ; Hiroshi Mori
• 关键词：Stx2b ; Heat-labile enterotoxin ; Mucosal adjuvant
• 刊名：Vaccine
• 出版年：2008
• 出版时间：24 January 2008
• 年：2008
• 卷：26
• 期：4
• 页码：469-476
• 全文大小：581 K

文摘

Escherichia coli O157:H7 produces Stx1 and Stx2 causing severe diseases. Their B subunits (StxBs) are useful for a vaccine but exhibit low immunogenicity, especially Stx2B. Nasal vaccination with StxBs plus cholera toxin induces only serum anti-Stx1B antibodies in mice. However, nasal administration of a mutant of E. coli enterotoxin and His-tagged Stx2B induced serum antibodies neutralizing Stx2 in vitro or in vivo and mucosal IgA antibodies in lungs. As His-tagged Stx2B showed five or three polymers in gel filtration chromatography, His-tagged Stx2B forms smaller tertiary structure than the native one and is effective for preventing Stx2 toxemia as a nasal vaccine.