A simple and rapid method of bacterial transformation

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• 作者：Gottfried Wilharm ; Daniela Lepka ; Franziska Faber ; Julia Hofmann ; Tobias Kerrinnes ; Evelyn Skiebe
• 刊名：Journal of Microbiological Methods
• 出版年：2010
• 出版时间：February 2010
• 年：2010
• 卷：80
• 期：2
• 页码：215-216
• 全文大小：218 K

文摘

Abstract

Recently, a unique method for bacterial transformation using nanofibers to inoculate DNA has been developed by Naoto Yoshida and colleagues. We have verified the principle, transforming Escherichia coli, Yersinia enterocolitica and Acinetobacter baumannii, and have established a user-friendly protocol. A buffered suspension of sepiolite—an inexpensive, fibrous yet inoffensive mineral—is mixed with bacteria and transforming DNA and the mixture directly spread on selective agar.