Solid-state fermentation for the production of biomass valorizing feruloyl esterase

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• 作者：Nishant Gopalan^a ; ^b ; Kesavan Madhavan Nampoothiri^a ; ^{madhavan85@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; George Szakacs^c ; Binod Parameswaran^a ; Ashok Pandey^a
• 关键词：AnFaeA ; Aspergillus niger feruloyl esterase Type A ; AtFaeA ; Aspergillus tubingensis feruloyl esterase Type ; AwFaeA ; Aspergillus awamori feruloyl esterase Type A ; CCD ; Central Composite Design ; DMSO ; Dimethyl Sulphoxide ; min ; minutes ; MOPS ; 3-(N-morpholino) propanesulfonic acid ; rpm ; Rotations per minute ; SMF ; Submerged fermentation ; SSF ; Solid state fermentation ; U/gds ; Enzyme units per gram dry substrate ; U/mL ; Enzyme units per milliliter
• 刊名：Biocatalysis and Agricultural Biotechnology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：7
• 期：Complete
• 页码：7-13
• 全文大小：1099 K

文摘

Aspergillus niger ATCC 13497 has been used to produce feruloyl esterase type A enzyme. The process parameters were optimized to attain a maximum of 11984.1 U/gds of enzyme activity within a period of 72 h using destarched wheat bran as a substrate. The purified enzyme studied in the present work displays a pH optimum of 5, and a temperature optimum of 37 °C. The enzyme has a size of 35 kDa and exhibited moderate thermostability, with 41% of activity retained at 90 °C. Ni ⁺² and Co⁺² ions were found to have stimulatory activity on the enzyme with 66% and 78% increase in activity respectively. Cu+2 inhibited the activity by 88%. Statistical optimization enhanced the enzyme activity by 2.47 fold.