Kinetics of heat- and acidification-induced aggregation of firefly luciferase

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• 作者：Wang ; Keyang ; Kurganov ; Boris I.
• 关键词：Luciferase ; Aggregation ; Kinetics ; Nucleation
• 刊名：Biophysical Chemistry
• 出版年：2003
• 出版时间：November 1, 2003
• 年：2003
• 卷：106
• 期：2
• 页码：97-109
• 全文大小：188 K

文摘

The general approach to analysis of the kinetics of protein aggregation registered by the turbidimetric method has been elaborated. The terminal part of the kinetic curves is analyzed using a theoretical equation connecting the derivative of the apparent absorbance (A) with respect to time (dA/dt) and A (t is time). This analysis allows the limiting value of A at t→∞ (A_lim) and the order of aggregation with respect to protein (n) to be calculated. Approach proposed was applied to analysis of thermal and acidification-induced aggregation of firefly luciferase. In both cases the A_lim value is a linear function of the protein concentration. The terminal part of the kinetic curves of thermal aggregation follows the first-order kinetics (n