Kinetics of functionalised carbon nanotube distribution in mouse brain after systemic injection: Spatial to ultra-structural analyses

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• 作者：Julie T.-W. Wang^a ; Noelia Rubio^a ; Houmam Kafa^a ; Enrica Venturelli^b ; Chiara Fabbro^c ; Cé ; cilia Mé ; nard-Moyon^b ; Tatiana Da Ros^c ; Jane K. Sosabowski^d ; Alastair D. Lawson^e ; Martyn K. Robinson^e ; Maurizio Prato^c ; Alberto Bianco^b ; Frederic Festy^f ; Jane E. Preston^a ; Kostas Kostarelos^g ; ^{k.kostarelos@ucl.ac.uk" class="auth_mail" title="E-mail the corresponding author} ; Khuloud T. Al-Jamal^a ; ^{khuloud.al-jamal@kcl.ac.uk" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Carbon nanotubes ; Brain drug delivery ; Blood&ndash ; brain barrier ; Nanomedicine ; SPECT/CT imaging ; Multi-photon luminescence microscopy
• 刊名：Journal of Controlled Release
• 出版年：2016
• 出版时间：28 February 2016
• 年：2016
• 卷：224
• 期：Complete
• 页码：22-32
• 全文大小：2220 K

文摘

Earlier studies proved the success of using chemically functionalised multi-walled carbon nanotubes (f-MWNTs) as nanocarriers to the brain. Little insight into the kinetics of brain distribution of f-MWNTs in vivo has been reported. This study employed a wide range of qualitative and quantitative techniques with the aim of shedding the light on f-MWNT's brain distribution following intravenous injection. γ-Scintigraphy quantified the uptake of studied radiolabelled f-MWNT in the whole brain parenchyma and capillaries while 3D-single photon emission computed tomography/computed tomography imaging and autoradiography illustrated spatial distribution within various brain regions. Raman and multiphoton luminescence together with transmission electron microscopy confirmed the presence of intact f-MWNT in mouse brain, in a label-free manner. The results evidenced the presence of f-MWNT in mice brain parenchyma, in addition to brain endothelium. Such information on the rate and extent of regional and cellular brain distribution is needed before further implementation into neurological therapeutics can be made.