In situ DIG-labeling, loop-mediated DNA Amplification (ISDL) for highly sensitive detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV)
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- 作者：Sarocha Jitrakorna ; b ; Narong Arunrutc ; Piyachat Sanguanrutb ; d ; Timothy W. Flegela ; b ; Wansika Kiatpathomchaic ; wansika@biotec.or.th" class="auth_mail" title="E-mail the corresponding author ; Vanvimon Saksmerpromea ; vanvimon.sak@biotec.or.th" class="auth_mail" title="E-mail the corresponding author
- 关键词：PstDNV ; IHHNV ; In situ LAMP ; In situ detection ; DIG-labeled ; Shrimp virus ; Digoxygenin
- 刊名：Aquaculture
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：456
- 期：Complete
- 页码：36-43
- 全文大小：4930 K
文摘
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ISDL is a highly sensitive method for IHHNV detection in shrimp tissues.
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ISDL gave no cross-reactions with tissues of shrimp infected with other pathogens.
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The total time used in ISDL was one-third of that needed for conventional ISH.
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Proteinase-K digestion step needed for normal ISH was unnecessary for ISDL.