Mapping of phospholipids by MALDI imaging (MALDI-MSI): realities and expectations
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- 作者：Louis J. Sparvero ; Andrew A. Amoscato ; C. Edward Dixon ; Joseph B. Long ; Patrick M. Kochanek ; Bruce R. Pitt ; H¨¹lya Bay?r ; Valerian E. Kagan
- 关键词：9-AA ; 9-aminoacridine ; ALI ; acute lung injury ; CA ; cornu ammonis ; CCI ; controlled cortical impact ; Cer ; ceramide ; CL ; cardiolipin ; CNS ; central nervous system ; DAG ; diacylglycerol ; DESI ; desorption electrospray ionization ; DHA ; docosahexaenoic acid ; DHB
- 刊名：Chemistry and Physics of Lipids
- 出版年：2012
- 出版时间：July, 2012
- 年：2012
- 卷：165
- 期：5
- 页码：545-562
- 全文大小：3526 K
文摘
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has emerged as a novel powerful MS methodology that has the ability to generate both molecular and spatial information within a tissue section. Application of this technology as a new type of biochemical lipid microscopy may lead to new discoveries of the lipid metabolism and biomarkers associated with area-specific alterations or damage under stress/disease conditions such as traumatic brain injury or acute lung injury, among others. However there are limitations in the range of what it can detect as compared with liquid chromatography-MS (LC-MS) of a lipid extract from a tissue section. The goal of the current work was to critically consider remarkable new opportunities along with the limitations and approaches for further improvements of MALDI-MSI. Based on our experimental data and assessments, improvements of the spectral and spatial resolution, sensitivity and specificity towards low abundance species of lipids are proposed. This is followed by a review of the current literature, including methodologies that other laboratories have used to overcome these challenges.